| Component | Amount (μl) |
|---|---|
| Sample from Step 40 | 13.5 |
| 5× TGIRT-III buffer | 4.4 |
| 100 mM DTT | 1 |
| 10 mM dNTP mixture | 1 |
| RNase inhibitor | 0.5 (20 U) |
| TGIRT-III enzyme | 0.9 (180 U) |
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