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. Author manuscript; available in PMC: 2022 May 13.
Published in final edited form as: Nat Protoc. 2021 Mar 26;16(4):2286–2307. doi: 10.1038/s41596-021-00501-9
Component Amount (μl)
One aliquot from Step 48 11.5
25 μM Read 1 PCR primer 0.5
25 μM Read 2 PCR primer (a distinct barcoded primer for each sample) 0.5
2× KAPA HiFi HotStart ReadyMix PCR kit 12.5