Quantitative nucleotide resolution profiling of RNA cytidine acetylation by ac4C-seq

. Author manuscript; available in PMC: 2022 May 13.

Published in final edited form as: Nat Protoc. 2021 Mar 26;16(4):2286–2307. doi: 10.1038/s41596-021-00501-9

Stage	Cycle number	Denature	Anneal	Extend
1	1	95 °C, 2 min	-	-
2	2-5	98 °C, 20 s	65 °C, 15 s	72 °C, 15 s
3	6-10	98 °C, 15 s	-	72 °C, 15 s
4	11	-	-	72 °C, 2 min