. Author manuscript; available in PMC: 2022 May 13.

Published in final edited form as: Biochem Pharmacol. 2008 Jul 4;76(5):608–619. doi: 10.1016/j.bcp.2008.06.019

Table 1 –

Sequences of the primers used in this study to mutate LdTopIB

Mutation		Primers (5′–3′)
R314A	FW	GAC CGC CTC GCC CTC GCC GTT GGT AAT GAG AAG
	RV	CTT CTC ATT ACC AAC GGC GAG GGC GAG GCG GTC
K352A	FW	TTC GAC TTC CTG GGC GCG GAC TCG ATC CGC TAC
	RV	GTA GCG GAT CGA GTC CGC GCC CAG GAA GTC GAA
R410A	FW	CTG CCA AGG TGT TCG CCA CCT ACA ATG CCT CCA TC
	RV	GAT GGA GGC ATT GTA GGT GGC GAA CAC CTT GGC AG
H453A	FW	GCC ATT CTG TGC AAC GCC CAG AAG TCC GTC TCG
	RV	CGA GAC GGA CTT CTG GGC GTT GCA CAG AAT GGC
Y222F	FW	CCA GTA AAA TCA ACT TCA TCG ACC CCC GTA TC
	RV	GAT ACG GGG GTC GAT GAA GTT GAT TTT ACT GG
D353	FW	GAC TTC CTG GGC AAG GCC TCG ATC CGC TAC CAG
	RV	CTG GTA GCG GAT CGA GGC CTT GCC CAG GAA GTC
N221S	FW	GGC ACC AGT AAA ATC AGC TAC ATC GAC CCC CGT
	RV	ACG GGG GTC GAT GTA GCT GAT TTT ACT GGT GCC