Figure 1.
Physiological and molecular analysis of leaf growth and senescence. (A) Phenotypic analysis of leaf growth and senescence in M3 leaves at 10 d, 15 d, 21 d, 28 d, 35 d and 42 d after sowing. (B) Chlorophyll content (chla, chlb and total chlorophyll (chl-ab) in M3 leaf at 10 d, 15 d, 21 d, 28 d, 35 d and 42 d. Data represent mean values (±SD; n = 4), and were analyzed using one-way ANOVA LSD test (p < 0.05). Bars with different letters are statistically different from each other. (C) Expression analysis of photosynthesis-associated gene (PhAG, RBCS) at 21 d, 28 d, 35 d and 42 d. (D) Expression analysis of senescence-associated genes (SAGs, NYE-1 and PaO) at 21 d, 28 d, 35 d and 42 d. Data in (C) and (D) represent mean values (±SD; n = 4) and were analyzed using Student’s t-test (NS; not significant, ** p < 0.01, *** p < 0.001) against 21 d time point.