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. 2022 May 6;11(9):1570. doi: 10.3390/cells11091570

Figure 4.

Figure 4

Physiological and molecular analysis of dark-induced leaf senescence. (A) Phenotype of M3 trifoliate leaves from 28-day-old plants at 0 d, or after an additional 1 d, 2 d, 3 d or 4 d of dark treatment (dD—days of dark treatment). (B) Chlorophyll concentration (Chl-a, Chl-b and total chlorophyll, Chl-ab) in M3 trifoliate leaf at 0 dD, 1 dD, 2 dD, 3 dD and 4 dD. Data represent mean values (±SD; n = 3) and were analyzed using one-way ANOVA LSD test (p < 0.05) and bars with different letters in (B) are statistically not similar to each other. Expression analysis of (C) photosynthesis-associated genes (PhAG, RBCS) and (D) senescence-associated genes (SAGs, NYE-1 and PaO), at 0 dD, 1 dD, 2 dD and 3 dD. Data in (C) and (D) represent mean values (±SD; n = 3), and were analyzed using Student’s t-test (*** p < 0.001) against 0 dD time point.