Figure 9.

Transient expression of putative senescence-associated transcription factors (SA-TFs). (A) Venn diagrams of senescence-induced TFs at different time points in age-dependent and dark-induced senescence. (B) Transient overexpression of ORE-1 and AtNAP (35S:TFs) in tobacco leaves through the agroinfiltration method. Expression of GFP under 35S promoter was used as a positive control for gene expression and negative control for senescence induction. (C) SA-TFs were cloned into expression vectors under 35S CaMV promoter and transiently expressed in tobacco leaves using the agroinfiltration method. Leaves were photographed six to seven days after infiltration.