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. 2022 May 9;23(9):5259. doi: 10.3390/ijms23095259

Figure 9.

Figure 9

The effect of proteosome inhibitor MG132 and lysosome inhibitor NH4Cl on the angiogenic abilities after FXR overexpression. (A) The human umbilical vein endothelial cells (HUVECs) were cultured with FXR overexpressed conditioned medium (CM) and control CM with or without MG132 or NH4Cl of bladder cancer cells for 6 h. The formation of an endothelial cell network was observed and the number of branch points and tube length in the T24 CM were analyzed. Scale bar = 200 μm. (B) VEGFA, p-STAT3, NOS2 and HIF-1α were analyzed by Western blotting in T24 cells with or without MG132 or NH4Cl. GAPDH was used as the loading control. The bar graphs show the relative attenuation of branch points and tube lengths. * p < 0.05, ** p < 0.01 compared with control group; # p < 0.05, ## p < 0.01; ### p < 0.001 compared with FXR overexpression group.