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. 2022 May 9;23(9):5259. doi: 10.3390/ijms23095259

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The effect of proteosome inhibitor MG132 on the migration after FXR overexpression CM in HUVECs. (A) Wound healing migration assays were performed in HUVECs after treatment with FXR overexpression CM with or without MG132. The lower panel displays quantitative results of the relative migration rate of the wound healing migratory abilities. *** p < 0.001 compared to the control group; ### p < 0.001 compared to the FXR-O group. Scale bar = 200 μm. (B,C) VEGFR1, VEGFR2, VEGFA, p-FAK, p-paxillin, p-MLC were analyzed by Western blotting in HUVECs with or without MG132. GAPDH was used as the loading control. The bar graphs show the relative quantitative analysis of the aforementioned proteins. * p < 0.05 compared with the control group; # p < 0.05; ### p < 0.001 compared to the FXR-O group.