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. 2022 Apr 19;11(9):1377. doi: 10.3390/cells11091377

Figure 1.

Figure 1

Schematic representation of Nr3c2 (MR) 3′-UTR, expression of Nr3c2 (MR) and miRNA candidates in renal KC3AC1 cells under hypertonicity. (A) Location of predicted binding sites for miRNAs in the murine Nr3c2 (MR) 3′-UTR, 2.78 kbp, positioned after the stop codon TGA, arbitrarily set at +1. (B,C) RT-qPCR analyses of renal MR and expression of miRNA candidates from TLDA (B) and miRNAs-seq (C) approaches. KC3AC1 cells were grown for 7 days in complete medium then cells were exposed to isotonicity (Iso) or hypertonicity (Hyper) for 6 h. Nr3c2 (MR) transcript and miRNA levels under hypertonicity are expressed as a percentage of Nr3c2 (MR) mRNA or miRNA levels under isotonicity (arbitrarily set at 100%). Data are means ± SEMs from three independent experiments performed in six replicates (n = 18); isotonic condition (open circle, o), hypertonic condition (black circle, •). NS = not significant, ** p < 0.01, *** p < 0.001, **** p < 0.0001.