Skip to main content
. 2022 Apr 19;11(9):1377. doi: 10.3390/cells11091377

Figure 6.

Figure 6

Impairment of MR signaling in KC3AC1 cells stably expressing miR-324-5p. miR-324-5p (A,D), Nr3c2 (MR) (B,E) and Elavl1 (HuR) (C,F) expression were determined by RT-qPCR, 48 h after doxycycline induction (1 µg/mL) in KC3AC1 clones stably transduced with lentiviral particles expressing inducible scrambled miRNAs (Sm-A1 clone, left panels) or miR-324-5p (Sh-H8 clone, right panels). Data are means ± SEMs from two independent experiments performed in six replicates (n = 12). NS = not significant, *** p < 0.001, **** p < 0.0001 compared to the condition in absence of doxycycline, arbitrarily set at 100%. (G,H) Sm-A1 and Sh-H8 clones were deprived in minimal medium for 48 h and incubated for 48 h with 1 µg/mL doxycycline. Thereafter, Sm-A1 and Sh-H8 clones were stimulated for 1 h with 10 nM aldosterone and Tsc22d3 (Gilz) expression (G,H) was quantified by RT-qPCR where basal Gilz expression in renal cells, in the absence of DOX and aldosterone, was arbitrarily set at 100%. Data are means ± SEMs from three independent experiments performed in six replicates per condition (n = 18). NS = not significant. ** p < 0.01 *** p < 0.001 **** p < 0.0001 compared to the condition without aldosterone stimulation, arbitrarily set at 100%. §§§ indicate p < 0.001 between the two conditions indicated by line.