Figure 2.

Hypoxia reduces the fat content of mice and promotes the expression of thermogenic genes in brown fat. (A–C) Subcutaneous fat, perirenal fat, and brown fat were isolated and weighed. Statistical analysis was performed by Student’s t-test (mean ± SEM, n = 8,) *** p < 0.005. (D) White fat and brown fat were examined by H&E staining at (20×) and representative images of gross brown fat are shown. (E–G) Brown fat was immuno-stained with UCP1 and PPARγ antibodies, respectively. The stained slides were quantified by ImageJ software and the significance was tested by Student’s t-test (mean ± SEM, n = 3). * p < 0.05, *** p < 0.005.