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. 2022 May 6;23(9):5209. doi: 10.3390/ijms23095209

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Epinephrine mediates the effect of hypoxia on the expression of modulators for metabolism in liver. (A) Liver extracts were blotted with antibodies against UCP1, ADR3, PKAc, P-AMPK, AMPK, and PGC1α. A representative set of blots from two independent experiments is presented. (B–F) The protein bands in (A) were quantified using Image J software. (G,H) Liver tissues were examined by immunohistochemical staining of CPT1A (40×) and the positive intensity was analyzed by Image J software (H). (I,J) Western blot analysis of CD206 was performed (I) and semi-quantification normalized with GAPDH (J). (K) qPCR analysis was performed on mRNA for ATGL, UCP2, C/EBP, Adiponectin, and PPARα using 18S rRNA as an internal control. Student’s t-test was used for all statistical analysis (mean ± SEM). * p < 0.05, ** p < 0.01.