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. 2022 May 10;10:e13206. doi: 10.7717/peerj.13206

Table 3. Effect of environmental conditions on seedling emergence percentage (EP) and emergence index (EI) of 10 alfalfa seed lots.

Seed lot Control Water stress Salinity stress
EP (%) EI EP (%) EI EP (%) EI
1 93.33 ± 2.40 Aa 7.15 ± 0.85 Aa 42.00 ± 2.00 Bab 1.64 ± 0.20 Ba 41.33 ± 4.67 Ba 1.38 ± 0.17 Ba
2 74.67 ± 7.68 Ab 4.25 ± 0.70 Abc 36.67 ± 0.67 Bb 1.36 ± 0.11 Ba 27.33 ± 2.67 Bb 0.87 ± 0.07 Bcd
3 54.67 ± 2.67 Ac 2.73 ± 0.46 Ac 23.33 ± 2.40 Bc 0.78 ± 0.06 Bb 16.67 ± 1.76 Bc 0.48 ± 0.03 Bd
4 90.00 ± 5.29 Aab 5.66 ± 0.74 Aab 42.67 ± 3.53 Bab 1.85 ± 0.31 Ba 36.00 ± 3.06 Bab 1.34 ± 0.19 Bab
5 85.33 ± 5.46 Aab 5.25 ± 0.39 Aab 46.00 ± 3.06 Ba 1.86 ± 0.13 Ba 32.00 ± 1.16 Cab 1.12 ± 0.02 Babc
6 90.67 ± 3.33Aa 6.49 ± 0.86 Aa 42.67 ± 2.67 Bab 1.90 ± 0.20 Ba 34.67 ± 3.53 Bab 1.40 ± 0.16 Ba
7 87.33 ± 1.76 Aab 5.57 ± 0.23 Aab 38.67 ± 2.91 Bab 1.48 ± 0.01 Ba 37.33 ± 2.40 Bab 1.30 ± 0.11 Bab
8 81.33 ± 7.33 Aab 4.02 ± 0.50 Abc 46.00 ± 4.00 Ba 1.63 ± 0.25 Ba 29.33 ± 4.06 Bb 0.93 ± 0.07 Bbc
9 88.00 ± 4.00 Aab 5.44 ± 0.42 Aab 40.67 ± 2.91 Bab 1.54 ± 0.07 Ba 28.00 ± 2.31 Cb 0.94 ± 0.12 Bbc
10 88.67 ± 3.33 Aab 5.70 ± 0.62 Aab 43.33 ± 1.33 Bab 1.69 ± 0.11 Ba 32.67 ± 3.71 Bab 1.15 ± 0.21 Babc
Source of variation DF EP EI
Chisq P Chisq P
Seed lot (SL) 9 109.933 <0.001 137.120 <0.001
Environmental conditions (EC) 2 73.432 <0.001 182.878 <0.001
SL × EC 18 36.683 0.006 64.685 <0.001

Note:

Different uppercase indicated significant difference among different stress treatments for given seed lot at the 0.05 level (P < 0.05), different lowercase indicated significant difference among different seed lots for given stress treatment at the 0.05 level (P < 0.05). The effect of seed lot and environmental conditions on seedling emergence percentage and emergence index were analyzed using generalized linear mixed models. Three replications for each treatment of each seed lot. Duncan’s test was used to compare means when significant differences were found.