TABLE 2.
Induction of QR by various aromatic compoundsa
| Inducer | Concn (mM) | Induction (fold)b |
|---|---|---|
| Quinones | ||
| p-Benzoquinone | 0.2 | 12 |
| MBQ | 0.2 | 12 |
| DMBQ | 0.2 | 12 |
| 1,4-Naphthaquinone | 0.2 | 10 |
| 3,5-Di-tert-buryl-1,2-orthoquinone | 0.2 | 1.3 |
| 2-Chloroquinone | 0.2 | 2.2 |
| Hydroquinones | ||
| p-Hydroquinone | 0.2 | 6.8 |
| 2-Methoxyhydroquinone | 0.2 | 11 |
| 2,6-Dimethoxyhydroquinone | 0.2 | 11 |
| Aromatic acids | ||
| Benzoic acid | 2.0 | 1 |
| p-Hydroxybenzoic acid | 2.0 | 8.8 |
| Vanillic acid | 2.0 | 25 |
| 3,4-Dihydroxybenzoic acid | 2.0 | 1.4 |
| 3,5-Dihydroxybenzoic acid | 2.0 | 1 |
| Syringic acid | 2.0 | 1.3 |
| Ferulic acid | 2.0 | 28 |
| Veratic acid | 2.0 | 4.0 |
| Aromatic aldehyde | ||
| Vanillyl aldehyde | 2.0 | 15 |
| Aromatic alcohols | ||
| Veratryl alcohol | 2.0 | 1 |
| Vanillyl alcohol | 2.0 | 1 |
| Phenols | ||
| Phenol | 0.2 | 1 |
| 4-Chlorophenol | 0.2 | 7.3 |
| 2,4-Dichlorophenol | 0.2 | 1 |
| 2,6-Dichlorophenol | 0.2 | 2.2 |
| 2,6-Dichlorophenol | 0.2 | 2.0 |
| 3,4-Dichlorophenol | 0.2 | 1.9 |
| 3,5-Dichlorophenol | 0.2 | 1 |
| 2,4,5-Trichlorophenol | 0.2 | 1.9 |
| 2,4,6-Trichlorophenol | 0.2 | 1 |
| Pentachlorophenol | 0.2 | 1 |
| Nitroaromatic compounds | ||
| Nitrobenzene | 0.2 | 1 |
| 2-Nitrophenol | 0.2 | 5.7 |
| 4-Nitrophenol | 0.2 | 1.8 |
| 2,4-Dinitrobenzoic acid | 0.2 | 1 |
| 3,5-Dinitrobenzoic acid | 0.2 | 1 |
| 2,4-Dinitrotoluene | 0.2 | 1 |
| 2,6-Dinitrotoluene | 0.2 | 1 |
| 4-Nitrocatechol | 0.2 | 1 |
| 2,4,6-Trinitrotoluene | 0.2 | 2.2 |
Cells were grown for 2 days in HN medium, and then compounds were added as described in the text; 16 h after the compounds were added, cells were harvested and broken, and the enzyme was assayed as described in the test.
The level of induction was determined by dividing the specific activity of an induced culture by the specific activity of the uninduced control.