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. 2022 Apr 26;23(9):4764. doi: 10.3390/ijms23094764

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Schematic representation of the main methods used for identifying alterations in the ECS. AEA: anandamide; 2-AG: 2-arachidonoylglycerol; CB1r: cannabinoid 1 receptor; CB2r: cannabinoid 2 receptor; DAGL: diacylglycerol lipase; EMT: endocannabinoid membrane transporter; FAAH: fatty acid amide hydrolase; GTPyS: GTPgammaS or guanosine 5′-O-(γ-thio) triphosphate; LC-MS / MS: liquid chromatography-tandem mass spectrometry; MAGL: monoacylglycerol lipase; NarPE: N-arachidonoyl phosphatidylethanolamine; NAPE-PLD: N-acylphosphatidylethanolamine specific phospholipase D; NAT: N-acyl-transferase; NGS: next-generation sequencing (genomics); PET: positron emission tomography; q-PCR: real-time polymerase chain reaction; SNPs: single nucleotide polymorphisms; TLC: thin-layer chromatography.