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. 2022 Apr 28;27(9):2802. doi: 10.3390/molecules27092802

Table 4.

Primers used in real-time PCR.

Gene Primer Sequence
HNF-1α F: 5′-GACCTGACCGAGTTGCCTAAT-3′
R: 5′-CCGGCTCTTTCAGAATGGGT-3′
Sirtuin-1 F: 5′- CCAGAACAGTTTCATAGAGCC-3′
R:5′-TCTTACTTTCAGAGAAGACCCAATA-3′
C/ebpα F:5′- CAAGAACAGCAACGAGTACCG-3′
R: 5′- GTCACTGGTCAACTCCAGCAC-3′
miRNA-34a F:5′--UGG CAG UGUCUU AGC UGG UUG UU-3′
R: 5′-CAA CCAGCU AAG ACA CUG CGA AA-3′.
miRNA-223 F: 5′-TGGATCCGTGTCACTCGGGCTTTACCTG-3′
R: 5′- CGAATTCGTAGACACAGCCCAGGGCTGT-3′.
β-Actin F:5′-GGCTGTATTCCCCTCCATCG-3′
R: 5′- CCAGTTGGTAACAATGCCATGT-3′