Figure 5.

IGF1 is a target gene of miR-222. (A₁–A₈) Expression signature of muscle atrophy marker genes and node genes (miR-222 target gene interaction network) in a denervated muscle atrophy model. Data were sourced from the GEO database (GSE77121, GSM2044533: Control diet_Sham tretment, GSM2044534: Control diet_Denervated treatment, GSM2044535: Genistein diet_Sham treatment, GSM2044536: Genistein diet_Denervated treatment) and analyzed online using the GEOR2 tool. (B–D) Prediction and secondary structure analysis of IGF1 and miR-222 binding sites. (E,F) Fluorescence intensity of a dual-luciferase reporter assay system for two potential binding sites of miR-222 and IGF1. (G) Correlation analysis of miR-222 and IGF1 expression levels in TA muscle. C, F_G, n = 3; H, n = 18. * p < 0.05, compared with the NC group.