Figure 1.

Concentration-dependent effect of methanol on survival fraction in two ovarian cancer cell lines. No significant reduction in survival fraction was observed in OVCAR-3 (blue) or Caov-3 (red) cells after 48 h incubation with ≤1% methanol (v/v) in complete cell culture medium (vehicle control for all subsequent experiments) (mean ± standard deviation (SD) expressed as a percentage of the no methanol control (dashed line); n = 3 independent experiments in duplicate). Significant differences between exposure group versus unexposed control are denoted by * (p < 0.05).