Table 3.

Screening analysis of the expression of the remaining genes associated with the function of the immune system. All results are expressed as a mean of 2^−ΔCt (according to the reference gene encoding 18S rRNA); N/A–not available. The values of each mean gene’s expression in the study group are marked in red, while in the control group in blue.

No.	Assay ID	Gene Symbol	Gene Expression (2−ΔCt) for Control	Gene Expression (2−ΔCt) for ACS
1	Hs00174092_m1	IL1A	N/A	N/A
2	Hs00174097_m1	IL1B	2.304	2.021
3	Hs00174114_m1	IL2	0.011	N/A
4	Hs00174117_m1	IL3	N/A	N/A
5	Hs00174122_m1	IL4	N/A	N/A
6	Hs00174200_m1	IL5	N/A	N/A
7	Hs00174202_m1	IL7	N/A	N/A
8	Hs00174103_m1	IL8	3.148	3.398
9	Hs00174125_m1	IL9	N/A	N/A
10	Hs00174086_m1	IL10	N/A	N/A
11	Hs00168405_m1	IL12A	0.026	0.015
12	Hs00233688_m1	IL12B	N/A	N/A
13	Hs00174379_m1	IL13	N/A	N/A
14	Hs00174106_m1	IL15	N/A	N/A
15	Hs00174383_m1	IL17A	N/A	N/A
16	Hs00155517_m1	IL18	0.034	0.029
17	Hs00171149_m1	CCL19	N/A	N/A
18	Hs00171041_m1	CXCR3	N/A	N/A
19	Hs00171042_m1	CXCL10	0.025	0.015
20	Hs00171138_m1	CXCL11	0.016	N/A
21	Hs00174164_m1	CSF1	0.007	0.005
22	Hs00357085_g1	CSF3	N/A	N/A
23	Hs00234174_m1	STAT3	0.630	0.362
24	Hs00174517_m1	NFKB2	0.315	0.324
25	Hs00233284_m1	IKBKB	0.065	0.035
26	Hs00167894_m1	CD3E	1.046	1.380
27	Hs00181217_m1	CD4	0.239	0.271
28	Hs00233520_m1	CD8A	0.509	0.530
29	Hs00174333_m1	CD19	0.057	0.014
30	Hs00166229_m1	IL2RA	0.085	0.021
31	Hs00174796_m1	CD28	0.130	0.115
32	Hs00233552_m1	CD38	0.074	0.031
33	Hs00365634_g1	PTPRC	3.397	2.818
34	Hs00154355_m1	CD68	1.370	0.653
35	Hs00175478_m1	CD80	0.009	0.007
36	Hs00199349_m1	CD86	0.443	0.347
37	Hs00175480_m1	CTLA4	0.063	0.070
38	Hs00163934_m1	CD40LG	0.133	0.017
39	Hs00219575_m1	HLA-DRA	7.697	9.746
40	Hs99999917_m1	HLA-DRB1	N/A	N/A
41	Hs00203436_m1	TBX21	0.057	0.049
42	Hs00188346_m1	TNFRSF18	N/A	N/A
43	Hs00167248_m1	NOS2	N/A	N/A
44	Hs00169141_m1	BCL2L1	0.431	0.239
45	Hs00180269_m1	BAX	0.410	0.244
46	Hs00164932_m1	ICAM1	0.111	0.135
47	Hs00174583_m1	SELP	0.218	0.110
48	Hs00174057_m1	SELE	N/A	N/A
49	Hs00157965_m1	HMOX1	0.194	0.055
50	Hs00189742_m1	LRP2	N/A	N/A
51	Hs00167927_m1	CYP1A2	N/A	N/A
52	Hs00167982_m1	CYP7A1	N/A	N/A
53	Hs00174143_m1	IFNG	0.008	0.010
54	Hs00246266_m1	GNLY	4.939	7.386
55	Hs00163653_m1	FAS	0.242	0.296
56	Hs00181225_m1	FASLG	N/A	N/A
57	Hs00171257_m1	TGFB1	2.304	2.403
58	Hs00232222_m1	SMAD3	0.035	0.037
59	Hs00178696_m1	SMAD7	0.075	0.035
60	Hs00365052_m1	FN1	N/A	N/A
61	Hs00163811_m1	C3	0.014	0.006
62	Hs00174128_m1	TNF	0.062	0.074
63	Hs00236874_m1	LTA	N/A	N/A
64	Hs00174179_m1	ACE	N/A	N/A
65	Hs00173626_m1	VEGFA	N/A	N/A
66	Hs00161707_m1	SKI	0.035	0.047
67	Hs00156373_m1	CD34	N/A	N/A
68	Hs00241341_m1	AGTR1	N/A	N/A
69	Hs00169126_m1	AGTR2	N/A	N/A
70	Hs00174961_m1	EDN1	0.043	N/A
71	Hs00171455_m1	LIF	N/A	N/A
72	Hs00209771_m1	LY96	0.043	0.618
73	Hs00236988_g1	MIF	6.889	5.080
74	Hs00190046_m1	NFATC3	1.664	1.735
75	Hs00190037_m1	NFATC4	N/A	N/A
76	Hs00236998_m1	PF4	18.692	14.772
77	Hs00374292_m1	SYK	N/A	N/A

For genes with N/A results we did not observe any product formation, thus the fluorescent level (RFU) did not exceed the threshold which was automatically calculated by the thermocycler during analysis.