Figure 1.

Microscopic image and schematic of the cube-shaped cell-trapping microwell: (A) Image of the channel in previous design with cube-shaped cell-trapping chamber. Each channel has a length of 78 mm with a cross-section of 600 μm × 600 μm with five cube-shaped cell-trapping chambers for cell sedimentation and spheroid formation. (B) Image of the modified design used in this work with cylindrical-shaped cell-trapping chambers. Each channel has a length of 109.2 mm with a cross-section of 840 μm × 840 μm with five cylindrical cell-trapping chambers for cell sedimentation and spheroid formation (C) Top view of microscopic image of cell-trapping chamber of the previous design; the size of the chamber is 600 μm × 600 μm and the height of the chamber is 540 μm. (D) Top view microscopic image of modified cylindrical-shaped cell-trapping chamber in the present study; the radius of the chamber is 840 μm and the height of the chamber is 756 μm. (E) Schematic top view of cube-shaped cell-trapping chamber in previous design. (F) Schematic top view of cylindrical-shaped cell-trapping chamber in the current design used in this work.