Figure 3.

Culture of different cell lines on PDMS biochips pre-coated with 3% BSA. Cell adhesion pattern to the surface is different, and it depends on the cell type. In some cell lines, like C4-2B, cell–substrate interactions are dominant over cell–cell interactions; therefore, they cannot form compact cell clusters on surfaces treated with 3% BSA. On the other hand, some cell lines like U251 demonstrated greater cell–cell interactions when compared with cell–substrate interactions; therefore, this leads to the formation of more compact cell clusters. The red color is due to red-fluorescent protein (RFP) in IRM-90 cells and the green color is due to green-fluorescent protein (GFP) in MDA-MB-231 cells. For other cell lines, the green color is due to being fluorescently labeled with cell-labeling solution Vybrant Dio V22886. Scale bars: 200 μm.