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. 2022 Apr 21;22(9):3191. doi: 10.3390/s22093191

Figure 4.

Figure 4

Proliferation and growth pattern for spheroid production on biochip pre-coated with 10% BSA. (A) Spheroid size over the seven days of culture. Spheroid size varied and was significantly increased on day 7 compared to day 1 for all cell lines (D1 vs. D7 ** p < 0.001 and *** p < 0.0001). C4-2B cell line was only able to produce spheroid after day 3 of culture, and we accordingly only saw the size on day 7. (B) BRL for U251, A549, and H1299 significantly increased on day 7 compared to day 1 (D1 vs. D7 * p < 0.05 and ** p < 0.001) and for IRM-90 and MDA-MB 231 varied non-significantly (NS = non-significant). (C) Circularity is representative of the spherical or circular shape of spheroids. Circularity increased non-significantly on day 7 compared to day 1 for all of the cell lines. C4-2B cell line only was able to produce spheroid after day 3 of culture, as previously mentioned. All assays were performed as triplicates per trial in three independent experiments. (Error bars represent ± SE, n = 3).