Figure 6.
MCTSs were produced from different ratios of tumor (A549) and stromal (IRM-90) cells. (A) Spheroid size on days 1, 3, and 7 of culture. In all three ratios between tumor and stromal cells, the size of the spheroids significantly changed on day 7 when compared to day 1 (D1 vs. D7 *** p < 0.0001). However, spheroids from different ratios of tumor–stromal cells (1:1, 1:2 and 2:1) on day 7 did not have a statistically significant difference in size. (B) Circularity of spheroids for the stromal–tumor ratio of IRM-90:A549 1:1 and IRM-90:A549 2:1 was increased significantly on day 7 when compared to day 1 (D1 vs, D7 * p < 0.05) and for IRM-90:A549 1:2 was not statistically significant (non-significant = NS). However, spheroids from different ratios of tumor–stromal cells (1:1, 1:2, and 2:1) on day 7 did not have a statistically significant difference in terms of circularity. (C) Probability of spheroid formation on-chip. By changing the ratio of tumor and stromal cell probability, spheroid production did not change statistically significantly. The total number of cells is 1 × 106 cells/mL for all three ratios. p < 0.05 is considered statistically significant. All assays were performed as triplicates per trial in three independent experiments. (Error bars represent ± SE, n = 3).