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. 2022 Apr 30;11(9):1320. doi: 10.3390/foods11091320

Table 1.

Enzymatic hydrolysis conditions for obtaining protein hydrolysates from bighead carp (Hypophthalmichthys nobilis) using ficin enzyme.

Hydrolysis Conditions Ficin Enzyme
Enzyme/substrate ratio (%) 1, 2, 3, 4, 5
Reaction pH 6
Temperature (°C) 40
Inactivation temperature of hydrolysis (°C) 70
Inactivation time (min) 20
Hydrolysis time (h) 1, 3, 6