Table 3.
Amino acid content of bighead carp protein hydrolysates (g/100 g protein) derived using ficin enzyme under optimal conditions.
| Amino Acids | Hydrolysates | ||
|---|---|---|---|
| Essential Amino Acids (EAAs) | DH 13.36% (1 h) | DH 17.09% (3 h) | DH 20.15% (6 h) |
| Histidine | 2.63 ± 0.02 b | 2.65 ± 0.02 b | 2.78 ± 0.05 a |
| Threonine | 3.13 ± 0.06 c | 3.24 ± 0.05 b | 3.36 ± 0.11 a |
| Valine | 4.23 ± 0.06 b | 4.32 ± 0.02 b | 4.43 ± 0.04 a |
| Methionine | 2.39 ± 0.16 b | 2.65 ± 0.02 a | 2.77 ± 0.10 a |
| Phenylalanine | 3.05 ± 0.02 c | 3.22 ± 0.01 b | 3.29 ± 0.02 a |
| Isoleucine | 3.80 ± 0.05 c | 3.86 ± 0.01 b | 4.01 ± 0.03 a |
| Leucine | 6.42 ± 0.05 c | 6.51 ± 0.02 b | 6.74 ± 0.02 a |
| Lysine | 8.12 ± 0.08 b | 8.01 ± 0.06 b | 8.49 ± 0.06 a |
| Arginine | 4.99 ± 0.01 b | 4.97 ± 0.01 b | 5.17 ± 0.07 a |
| Tyrosine | 2.03 ± 0.22 c | 2.34 ± 0.05 b | 2.62 ± 0.03 a |
| Nonessential amino acids (NAAs) | |||
| Cystenie | 0.23 ± 0.03 b | 0.28 ± 0.01 b | 0.36 ± 0.02 a |
| Aspartic acid | 9.72 ± 0.07 c | 10.00 ± 0.03 b | 10.45 ± 0.19 a |
| Glutamic acid | 13.79 ± 0.25 c | 14.02 ± 0.05 b | 14.46 ± 0.10 a |
| Serine | 2.26 ± 0.04 c | 2.59 ± 0.05 b | 2.70 ± 0.04 a |
| Glycine | 4.33 ± 0.07 a | 4.21 ± 0.04 b | 4.09 ± 0.01 c |
| Proline | 2.48 ± 0.02 a | 2.54 ± 0.04 a | 2.28 ± 0.02 b |
| Alanine | 4.70 ± 0.12 b | 4.91 ± 0.08 a | 5.01 ± 0.07 a |
| TEAA | 40.81 ± 0.45 c | 41.79 ± 0.29 b | 43.69 ± 0.36 a |
| TNAA | 37.52 ± 0.17 c | 38.57 ± 0.11 b | 39.38 ± 0.82 a |
| TAA | 78.33 ± 1.12 c | 80.36 ± 0.73 b | 83.07 ± 0.68 a |
| TEAA/TAA% | 51.97 ± 0.28 | 52.04 ± 0.41 | 52.85 ± 0.15 |
Different letters indicate that values are significantly different (p ≤ 0.05); values represent the mean of triplicate determinations, mean ± SD (n = 3). TAA, total amino acids; TEAA, total essential amino acids; TNAA, total nonessential amino acids; TEAA/TAA %, total essential amino acids to total amino acids.