FIG. 1.

Detection of MAL transporter genes in brewing strains of yeast. (A) Separation of chromosomes of yeast strains by PFGE. (B) Detection of MAL transporter genes after chromosomes were blotted onto a nylon membrane and hybridized with a probe for MAL61. Images were obtained by scanning with a Color OneScanner 600/27 scanner operated from an Apple Macintosh and were annotated in Adobe Photoshop 3.0 (Macintosh version). The Roman numerals on the left in panel A are the chromosome numbers, in order of decreasing size where more than one numeral is given on a line. The values on the right in panel A are the sizes (in kilobase pairs) of selected chromosomes. The positions of chromosomes carrying MAL11, MAL21, MAL31, and MAL41 are indicated on the right in panel B. Bands a and b are bands for specific chromosomes, as described in the text. Lanes 1 and 12, markers; lanes 2, KVL011; lanes 3, KVL012; lanes 4, KVL001; lanes 5, KVL004; lanes 6, KVL005; lanes 7, KVL006; lanes 8, KVL018; lanes 9, KVL021; lanes 10, KVL024; lanes 11, KVL026.