FIG. 2.

Detection of the AGT1 gene and homologues in brewing strains of yeast. (A) Separation of chromosomes of yeast strains by PFGE. (B) Detection of AGT1 after chromosomes were blotted onto a nylon membrane and hybridized with a probe for AGT1. Images were obtained by scanning with a Color OneScanner 600/27 scanner operated from an Apple Macintosh and were annotated in Adobe Photoshop 3.0 (Macintosh version). The Roman numerals on the left in panel A are the chromosome numbers, in order of decreasing size where more than one numeral is given on a line. The values on the right in panel A are the sizes (in kilobase pairs) of selected chromosomes. The position of chromosome VII carrying AGT1 is indicated on the right in panel B; bands a, b, and c are bands for specific chromosomes, as described in the text. Lanes 1 and 12, markers; lanes 2, KVL011; lanes 3, KVL012; lanes 4, KVL013; lanes 5, KVL014; lanes 6, KVL015; lanes 7, KVL024; lanes 8, KVL001; lanes 9, KVL006; lanes 10, KVL021; lanes 11, KVL026.