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. 2022 Apr 23;23(9):4689. doi: 10.3390/ijms23094689

Figure 2.

Figure 2

OSM induces inhibitory phosphorylation of IRS1 at serine 612 (S612). 3T3-L1 adipocytes were exposed to 0.5 nM OSM for 2 h, 8 h, or overnight (~16 h). Cells were harvested after OSM exposure, and lysates were subjected to immunoblotting for phospho-IRS1 (S612) and total IRS1; STAT5A was used as a loading control. OSM stimulated inhibitory IRS1 phosphorylation of S612 at all time points depicted. The immunoblot pictured here represents results from two independent experiments, each with 3 wells per condition (n = 6 total wells per condition).