TABLE 3.
Effects of various enzyme and chemical treatments on adhesion of R. toruloides to polystyrene
| Enzyme or chemical | Adhesiona |
|---|---|
| Snail gut enzyme | ++ |
| Purified glucuronidase | +++ |
| Sulfatase | ++++ |
| Mannosidase | +++ |
| Glucosidase | ++++ |
| Chitinase | ++++ |
| Protease | ++++ |
| Pronase E | ++++ |
| β-Mercaptoethanol | ++++ |
| Tunicamycin | + |
| NaOH (1 N) | − |
| HCl (1 N) | ++++ |
| Ethanol | − |
| Buffers | ++++ |
a
−, no adhesion; +, >60% reduction; ++, 30 to 60% reduction; +++, <30% reduction; ++++, no effect. The reduction in adhesion was significant at P = 0.05.