Figure 3.

Metformin therapy was associated with a reduction in the number of T-regs. (A) Representative confocal microscopy immunofluorescent images of metformin-treated and non-metformin-treated human samples (10×). (B) Quantification of immunologic phenotypes across tumor islet and tumor stromal segments as well as total cores. Phenotypes counted included CD4⁺/Foxp3⁻ (Th-cells), CD4⁺/Foxp3⁺ (T-regs), CD8⁺ (Tc-cells), CD20⁺ (B-cells), CD68⁺ (Macrophages), and CK⁺ (Tumor Cells). * indicates statistical significance.