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. 2022 Apr 28;23(9):4910. doi: 10.3390/ijms23094910

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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ELISA results for LTB4, IL-1β, TNF-α, IL-10, and TGF-β from the cell culture supernatant. (a) ELISA results for LTB4. The concentration of LTB4 in the haemolysate + vehicle group was higher than that in the control group. Following treatment with 5, 10, 15, and 20 μΜ of zileuton, the haemolysate-exposed BV-2 cells showed a lower concentration of LTB4 than those treated with the vehicle. There was no significant difference between haemolysate-exposed, zileuton-treated BV-2 cells, and BV-2 cells in the control group (*** p < 0.001 compared with control; ^## p < 0.01, ^### p < 0.001 compared with haemolysate + vehicle group, n = 3). (b) ELISA results for IL-1β. The haemolysate + vehicle group and the haemolysate + zileuton (5, 10, 15, and 20 μΜ) groups had a higher concentration of IL-1β than the control group. The haemolysate-exposed BV-2 cells treated with 10, 15, and 20 μΜ of zileuton displayed a lower concentration of IL-1β than BV-2 cells in the haemolysate + vehicle group (*** p < 0.001 compared with control; ^# p < 0.05, ^### p < 0.001 compared with haemolysate + vehicle group, n = 3). (c) ELISA results for TNF-α. The haemolysate-exposed BV-2 cells treated with the vehicle, as well as 5, 10, 15, and 20 μΜ of zileuton, showed elevated concentrations of TNF-α compared with the control group. The haemolysate-exposed BV-2 cells treated with 15 and 20 μΜ of zileuton showed a lower concentration of TNF-α than those treated with the vehicle (*** p < 0.001 compared with control; ^### p < 0.001 compared with haemolysate + vehicle group, n = 3). (d) ELISA results for IL-10. The haemolysate-exposed BV-2 cells treated with the vehicle, as well as 5, 10, 15, and 20 μΜ of zileuton, had an increased concentration of IL-10 compared with the control group. After being treated with 10, 15, and 20 μΜ of zileuton, haemolysate-exposed BV-2 cells showed a higher concentration of IL-10 than those treated with the vehicle (*** p < 0.001 compared with control; ^# p < 0.05, ^## p < 0.01, ^### p < 0.001 compared with haemolysate + vehicle group, n = 3). (e) ELISA results for TGF-β. The haemolysate-exposed BV-2 cells treated with the vehicle, as well as 5, 10, 15, and 20 μΜ of zileuton, showed an elevated concentration of TGF-β compared with the control group. Additionally, the concentration of TGF-β was higher in the haemolysate-exposed BV-2 cells treated with 10, 15, and 20 of zileuton than in those treated with the vehicle (*** p < 0.001 compared with control; ^### p < 0.001 compared with haemolysate + vehicle group, n = 3).