Table 2.
Comparison of compound treatment on the expression of selected genes in germinating Lepidium sativum (Lesa) seeds. Normalised RT-qPCR ratios (compound/control at the times indicated) for myrigalone A (MyA), MyB, MyD and angelicin treatments. Mean ± SEM from RNA extracted from three biological replicate plates.
| Gene Name | Control 12 h/Con 12 h | MyA b 12 h/Con 12 h | MyA b 18 h/Con 12 h | MyB b 12 h/Con 12 h | MyD b 12 h/Con 12 h | Angelicin c 12 h/Con 12 h | Angelicin c 18 h/Con 12 h |
|---|---|---|---|---|---|---|---|
| LesaGSTU25 | 1.0 ± 0.1 | 23.1 ± 12.7 | 49.0 ± 15.4 | 0.3 ± 0.2 | 2.5 ± 2.1 | 0.5 ± 0.3 | 0.2 ± 0.1 |
| LesaNAC102 | 1.0 ± 0.1 | 14.6 ± 4.9 | 4.1 ± 0.7 | 0.3 ± 0.1 | 0.5 ± 0.3 | 0.4 ± 0.1 | 0.5 ± 0.1 |
| LesaUGT73B5 | 1.0 ± 0.2 | 12.4 ± 3.9 | 8.7 ± 0.8 | 1.0 ± 0.2 | 1.2 ± 0.4 | 0.6 ± 0.1 | 0.6 ± 0.1 |
| LesaERF2 | 1.0 ± 0.2 | 6.6 ± 2.8 | 0.5 ± 0.1 | 0.3 ± 0.2 | 2.0 ± 0.9 | 0.3 ± 0.2 | 0.2 ± 0.1 |
| LesaABCG37 | 1.0 ± 0.2 | 5.2 ± 0.3 | 3.1 ± 0.3 | 0.9 ± 0.3 | 1.0 ± 0.2 | 0.5 ± 0.2 | 1.2 ± 0.4 |
| LesaOXI1 | 1.0 ± 0.4 | 4.8 ± 2.3 | 0.8 ± 0.1 | 0.7 ± 0.5 | 0.8 ± 0.2 | 0.3 ± 0.1 | 0.3 ± 0.1 |
| LesaCYP81D8 | 1.0 ± 0.3 | 4.4 ± 2.2 | 0.2 ± 0.1 | 0.2 ± 0.1 | 0.6 ± 0.3 | 0.4 ± 0.1 | 0.1 ± 0.0 |
| LesaOPR1/2 | 1.0 ± 0.1 | 4.3 ± 1.1 | 4.4 ± 0.1 | 1.6 ± 0.5 | 1.4 ± 0.2 | 0.9 ± 0.1 | 1.0 ± 0.1 |
| LesaWRKY75 | 1.0 ± 0.1 | 3.6 ± 1.1 | 2.9 ± 0.3 | 0.6 ± 0.2 | 0.6 ± 0.1 | 0.5 ± 0.1 | 0.8 ± 0.2 |
| LesaPER13 | 1.0 ± 0.2 | 3.4 ± 0.5 | 1.1 ± 0.2 | 0.9 ± 0.1 | 0.7 ± 0.1 | 0.5 ± 0.1 | 0.6 ± 0.2 |
| LesaWRKY23 | 1.0 ± 0.1 | 2.9 ± 1.5 | 0.9 ± 0.1 | 1.0 ± 0.5 | 1.7 ± 0.9 | 0.6 ± 0.0 | 0.6 ± 0.1 |
| LesaNAC005 | 1.0 ± 0.5 | 2.8 ± 1.5 | 6.5 ± 1.8 | 1.8 ± 0.6 | 1.7 ± 0.3 | 1.0 ± 0.3 | 3.6 ± 0.7 |
| LesaUGT74E2 | 1.0 ± 0.3 | 2.7 ± 0.7 | 0.7 ± 0.1 | 0.4 ± 0.3 | 0.4 ± 0.1 | 0.4 ± 0.1 | 0.1 ± 0.0 |
| LesaABCB4 | 1.0 ± 0.2 | 2.3 ± 1.2 | 1.5 ± 0.2 | 0.8 ± 0.1 | 1.2 ± 0.2 | 0.8 ± 0.2 | 1.2 ± 0.3 |
| LesaAOX1A | 1.0 ± 0.1 | 2.2 ± 1.3 | 0.5 ± 0.1 | 0.2 ± 0.0 | 0.6 ± 0.4 | 0.3 ± 0.1 | 0.3 ± 0.2 |
| LesaCYP81D4 | 1.0 ± 0.3 | 2.2 ± 0.2 | 0.4 ± 0.1 | 1.0 ± 0.4 | 0.9 ± 0.4 | 0.9 ± 0.2 | 0.2 ± 0.1 |
| LesaUGT75B1 | 1.0 ± 0.3 | 2.1 ± 1 | 0.5 ± 0.1 | 1.5 ± 1.0 | 0.9 ± 0.2 | 0.4 ± 0.0 | 0.4 ± 0.2 |
| LesaUGT75D1 | 1.0 ± 0.0 | 1.9 ± 0.2 | 1.6 ± 0.1 | 0.4 ± 0.1 | 0.7 ± 0.2 | 0.6 ± 0.1 | 1.1 ± 0.5 |
| Monooxygenase (AT5G64250) a | 1.0 ± 0.1 | 1.7 ± 0.2 | 0.9 ± 0.1 | 0.8 ± 0.1 | 1.0 ± 0.2 | 0.9 ± 0.1 | 0.4 ± 0.1 |
| LesaABCG34 | 1.0 ± 0.2 | 1.6 ± 0.4 | 1.5 ± 0.2 | 0.8 ± 0.3 | 0.8 ± 0.2 | 0.9 ± 0.1 | 1.5 ± 0.8 |
| LesaGSTU19 | 1.0 ± 0.1 | 1.5 ± 0.1 | 0.6 ± 0.1 | 0.9 ± 0.3 | 0.9 ± 0.3 | 0.8 ± 0.1 | 0.3 ± 0.1 |
| Oxidoreductase (AT3G44190) a | 1.0 ± 0.1 | 1.5 ± 0.2 | 4.3 ± 0.7 | 1.1 ± 0.0 | 1.3 ± 0.2 | 1.1 ± 0.2 | 0.7 ± 0.1 |
| LesaFSD1 | 1.0 ± 0.0 | 1.5 ± 0.1 | 5.5 ± 1.6 | 0.6 ± 0.1 | 0.7 ± 0.3 | 0.5 ± 0.1 | 4.1 ± 1.0 |
| LesaPER70 | 1.0 ± 0.4 | 0.6 ± 0.2 | 0.4 ± 0.1 | 1.1 ± 0.2 | 0.6 ± 0.2 | 0.3 ± 0.1 | 0.7 ± 0.2 |
| LesaTAT2 | 1.0 ± 0.1 | 0.6 ± 0.1 | 0.4 ± 0.0 | 0.4 ± 0.1 | 0.9 ± 0.3 | 0.7 ± 0.1 | 0.5 ± 0.2 |
| LesaPER45 | 1.0 ± 0.5 | 0.6 ± 0.3 | 1.6 ± 0.6 | 0.3 ± 0.2 | 0.9 ± 0.3 | 0.3 ± 0.1 | 3.2 ± 1.0 |
| LesaARF11 | 1.0 ± 0.3 | 0.5 ± 0.2 | 0.7 ± 0.2 | 1.4 ± 0.5 | 1.2 ± 0.2 | 0.9 ± 0.2 | 1.1 ± 0.4 |
| LesaCYP78A7 | 1.0 ± 0.1 | 0.4 ± 0.1 | 0.2 ± 0.0 | 0.8 ± 0.1 | 0.7 ± 0.1 | 0.9 ± 0.2 | 0.3 ± 0.1 |
| LesaUMAMIT25 | 1.0 ± 0.2 | 0.4 ± 0.1 | 0.3 ± 0.0 | 0.5 ± 0.1 | 0.6 ± 0.2 | 0.3 ± 0.1 | 0.4 ± 0.2 |
| LesaPIN7 | 1.0 ± 0.1 | 0.4 ± 0.1 | 0.3 ± 0.1 | 0.5 ± 0.2 | 0.6 ± 0.2 | 0.5 ± 0.1 | 0.7 ± 0.3 |
| LesaLHT1 | 1.0 ± 0.2 | 0.3 ± 0.1 | 0.3 ± 0.0 | 0.5 ± 0.0 | 0.6 ± 0.2 | 0.3 ± 0.1 | 1.1 ± 0.2 |
| LesaSKS15 | 1.0 ± 0.0 | 0.3 ± 0.2 | 2.2 ± 0.7 | 0.6 ± 0.1 | 0.9 ± 0.3 | 0.6 ± 0.2 | 3.5 ± 0.4 |
a putative Lesa orthologs of the AGIs: monooxygenase (AT5G64250), oxidoreductase (AT3G44190); b 0.5 mM; c 0.1 mM.