Table 4.
Comparison of compound treatment on the expression of selected detoxification, transporter and other genes. Results from myrigalone A (MyA) treated germinating Lepidium sativum seeds were compared to seeds, seedlings or roots cultures of Arabidopsis thaliana treated with tCHC, NCS, citral, BOA, PPA1, FEN, CMMP, MTX, DNP. Transcript abundance ratios compound/control ≥2 or ≤0.5 were considered as UP or DOWN (DN), respectively, or otherwise labelled as not at least 2-fold regulated (“-”). See Table 3 for abbreviations, references, and further details.
| Ratio MyA/Con | Ratio Compound/Control: ≥2 (UP), ≤2 (DN; Down) or Not 2-Fold Regulated (“-”) | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Myrigalone A | MyA | tCHC | tCHC | NCS | Citral | Citral | BOA | PAA1 | FEN | CMPP | MTX | DNP | |||
| AGI | Gene Name | 12/12 h | 18/12 h | Seeds | Roots | Shoots | Roots | Roots | Shoots | Seedlings | Seedlings | Root | Root | Seeds | Seeds |
| Cult. | Cult. | ||||||||||||||
| GST, peroxidase, glutathione and ascorbate system genes (substrate) | |||||||||||||||
| AT1G17180 | GSTU25 | 7.5 | 35.3 | UP | UP | UP | UP | - | - | UP | UP | UP | - | - | - |
| AT1G78380 | GSTU19 (OPDA) | 2.5 | 2.0 | UP | UP | UP | - | - | UP | - | UP | UP | UP | - | UP |
| AT1G78340 | GSTU22 | 3.6 | 8.8 | UP | UP | UP | UP | DN | - | UP | UP | - | UP | - | - |
| AT2G29490 | GSTU1 | 5.2 | 7.1 | UP | UP | UP | UP | DN | UP | UP | - | UP | UP | - | UP |
| AT3G09270 | GSTU8 | 17.2 | 30.9 | UP | - | UP | - | DN | - | UP | - | UP | UP | DN | DN |
| AT2G31570 | GPX2 | 1.0 | 2.6 | UP | - | - | - | DN | - | - | - | - | - | - | - |
| AT1G75270 | DHAR2 | 4.9 | 8.0 | UP | UP | UP | - | - | UP | - | UP | UP | UP | - | UP |
| AT1G07890 | APX1 | 1.0 | 2.4 | UP | - | - | - | DN | - | - | - | - | - | - | DN |
| AT4G35970 | APX5 | 1.1 | 4.8 | UP | - | - | - | DN | - | - | - | - | - | DN | DN |
| AT1G77100 | PER13 | 2.6 | 1.7 | UP | - | - | - | - | - | - | - | - | - | - | - |
| AT4G30170 | PER45 | 0.7 | 5.0 | UP | DN | - | DN | DN | - | DN | - | - | DN | DN | DN |
| AT4G25100 | FSD1 | 1.0 | 6.9 | UP | - | - | - | DN | DN | - | - | - | - | - | - |
| AT3G22370 | AOX1A | 2.3 | 3.3 | UP | - | - | UP | - | - | - | - | UP | UP | UP | UP |
| CYP750 and UGT protein genes (substrate) | |||||||||||||||
| AT4G37330 | CYP81D4 | 2.2 | 0.9 | UP | - | - | - | - | - | - | - | - | - | - | - |
| AT4G37370 | CYP81D8 | 5.3 | 5.6 | UP | UP | - | UP | UP | UP | UP | - | UP | UP | UP | UP |
| AT3G26290 | CYP71B26 | 0.9 | 3.1 | UP | - | DN | - | - | - | - | - | - | - | - | - |
| AT3G20950 | CYP75A32 | 1.2 | 5.3 | UP | - | - | - | DN | - | - | - | - | - | UP | DN |
| AT2G45570 | CYP76C2 | 2.5 | 3.7 | UP | - | - | - | - | UP | - | - | - | - | DN | DN |
| AT4G15550 | UGT75D1 (IBA) | 1.6 | 2.7 | UP | - | UP | - | - | UP | - | UP | UP | - | - | UP |
| AT1G05680 | UGT74E2 (IBA) | 2.4 | 3.1 | UP | - | - | UP | UP | UP | UP | - | UP | UP | UP | UP |
| AT1G05560 | UGT75B1 (IBA) | 2.6 | 2.2 | UP | - | UP | UP | UP | UP | UP | UP | UP | - | - | UP |
| AT2G15480 | UGT73B5 | 10.3 | 13.1 | UP | - | UP | UP | UP | UP | UP | - | UP | UP | - | UP |
| AT4G34138 | UGT73B1 | 1.7 | 2.3 | UP | - | UP | - | - | UP | UP | - | UP | UP | DN | UP |
| MATE, ABC, UMAMIT and aquaporin transporter (* see Table 3 for hormone-specific ABC transporter) | |||||||||||||||
| AT1G71140 | DTX14 | 3.2 | 1.4 | UP | UP | - | UP | UP | - | - | UP | - | UP | - | UP |
| AT4G25640 | DTX35 | 1.3 | 2.1 | UP | - | UP | - | - | - | - | - | UP | UP | - | - |
| AT1G66760 | DTX9 | 1.1 | 2.9 | UP | UP | - | - | - | DN | - | - | - | - | - | - |
| AT1G33100 | DTX2 | 1.8 | 2.8 | UP | - | - | - | DN | - | - | - | - | - | - | - |
| AT5G52450 | DTX16 | 1.1 | 3.0 | UP | - | - | UP | DN | - | - | - | - | - | - | - |
| AT2G36380 | ABCG34 * | 1.5 | 2.7 | UP | - | UP | - | DN | - | - | - | - | - | - | UP |
| AT4G01450 | UMAMIT30 | 1.4 | 2.7 | UP | - | - | DN | DN | - | - | - | - | - | - | - |
| AT4G28040 | UMAMIT33 | 1.1 | 3.3 | UP | UP | UP | DN | DN | DN | - | - | - | - | DN | - |
| AT4G30420 | UMAMIT34 | 2.2 | 8.8 | UP | - | - | - | DN | - | - | - | - | - | - | - |
| AT3G56620 | UMAMIT10 | 1.6 | 5.1 | UP | - | - | - | DN | - | - | - | - | - | UP | - |
| AT3G26520 | TIP1;2 | 0.9 | 2.1 | UP | - | - | DN | DN | DN | - | - | - | - | DN | DN |
| AT1G73190 | TIP3;1 | 2.7 | 1.4 | UP | - | - | - | - | - | - | - | - | - | UP | UP |
| AT1G01620 | PIP1;3 | 0.9 | 2.1 | UP | - | - | DN | DN | - | - | - | - | - | - | - |
| AT2G16850 | PIP2;8 | 1.0 | 3.4 | UP | - | - | - | - | DN | - | - | - | - | - | - |
| AT2G37170 | PIP2;2 | 0.8 | 2.2 | UP | DN | - | DN | DN | - | - | - | - | - | - | - |
| AT2G39010 | PIP2;6 | 1.7 | 4.2 | UP | - | - | DN | - | DN | - | - | - | - | DN | - |
| Transcription factors | |||||||||||||||
| AT1G02250 | NAC005 | 3.9 | 9.5 | UP | - | - | - | DN | - | - | - | - | - | - | - |
| AT1G77450 | NAC032 | 3.1 | 2.8 | UP | UP | UP | - | DN | UP | - | - | UP | UP | - | UP |
| AT5G08790 | NAC081/ATAF2 | 2.1 | 1.5 | UP | UP | - | UP | UP | - | - | - | - | - | - | - |
| AT5G63790 | NAC102 | 3.8 | 3.9 | UP | UP | UP | UP | - | UP | UP | UP | - | UP | - | UP |
| AT1G71520 | ERF20 | 12.4 | 8.3 | UP | UP | - | UP | UP | UP | - | - | - | - | - | - |
| AT1G74930 | ERF18/ORA47 | 7.0 | 4.0 | UP | - | - | DN | - | DN | - | - | - | - | - | - |
| AT3G23230 | ERF98/TDR1 | 5.1 | 8.3 | UP | - | - | - | - | - | - | - | - | - | - | - |
| AT5G47220 | ERF2 | 2.9 | 1.8 | UP | - | - | - | - | DN | - | - | - | - | - | UP |
| Other genes | |||||||||||||||
| AT1G76680 | OPR1/2 | 8.0 | 9.9 | UP | UP | - | UP | UP | UP | UP | UP | UP | UP | - | UP |
| AT5G22140 | Oxidoreductase | 27.9 | 49.4 | UP | UP | - | UP | UP | UP | - | - | - | - | - | - |
| AT3G44190 | Oxidoreductase | 91 | 349 | UP | UP | - | - | - | UP | - | - | UP | - | - | - |
| AT4G13180 | Oxidoreductase | 6.4 | 43.5 | UP | UP | UP | - | - | - | UP | UP | UP | UP | DN | UP |
| AT1G55920 | SAT1 | 4.7 | 5.6 | UP | UP | - | UP | - | UP | UP | UP | UP | UP | - | UP |
| AT5G39050 | PMAT1 | 2.0 | 2.6 | UP | UP | UP | UP | UP | UP | UP | - | UP | UP | - | UP |
| AT4G01870 | tolB | 6.7 | 5.3 | UP | UP | - | UP | UP | UP | UP | UP | UP | UP | - | UP |
| AT4G24160 | α/β-hydrolase | 2.0 | 1.5 | UP | UP | - | UP | - | UP | UP | - | UP | UP | - | UP |
| AT1G64670 | BDG1 (α/β-hyd.) | 1.0 | 3.0 | - | - | - | - | - | - | - | - | - | - | - | - |
| AT1G06570 | HPPD | 1.1 | 0.9 | - | UP | - | - | - | DN | - | - | - | - | - | UP |