Figure 3.

Photobleaching recovery for (a) fluorescence intensity ($I_{TA}\propto C_{TA}$) against r plotted from t = 0, immediately after photobleaching is fitted with Equation (22) for determining the value of R₀ and $I_{TB}$. (b) D_eff is determined by fitting the dimensionless average fluorescence intensity data ${\theta }_{Avg}$ against time by fitting to Equation (31) and $\beta$ is determined by measuring D in nonbinding system. Reprinted with permission from [95]. Copyright 1991, Elsevier.