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The overall optical train for the TIR/FRAP setup attached to an inverted microscope. The basic equipment is a 4 W argon ion laser, a low-light level-cooled CCD camera, an inverted microscope, and a computer for image capture and analysis. Beam Splitter: BS; Mirror: M; Dichroic Mirror: DM; Aperture: A; Prism: P; Shutter: S; Band Pass Filter: BF; Neutral Density Filler: NDF.
