Table 1.

Comparisons of various types of experimental methods for the measurement of biomolecular kinetics and/or transport processes.

Types of Biophysical Techniques	Measurable Processes	Limitations	References
Fluorescence Recovery After Photobleaching	Convection	Require sophisticated models;	[13]
	Diffusion	require high-powered lasers;	[45]
	Reaction/Binding	only valid for large ROI *	[60]
Fluorescence Correlation Spectroscopy	Diffusion	Lack of interpreting models;	[56]
	Reaction/Binding Concentration	difficult to apply in live cells; require high S/N * ratio	[61] [62]
Single Particle Tracking	Diffusion	Only for dilute species;	[22]
	Viscosity	measure lower mobility;	[55]
	Molecular Binding	requires feedback tracking	[63]
Surface Plasmon Resonance Sensor	Reaction/Binding	Require gold substrate; lack of	[52]
	Mass transfer	interpreting models; noise from used optoelectronics	[64] [65]
Stochastic Optical Reconstruction	Diffusion	High costs; require photo-	[66]
	Molecular Binding	switchable dyes as labels; require experts in operation	[67] [68]
	Molecular Interaction	Complicated mathematical	[57]
FCCS #	Composition/Fraction Large Complex	modeling; supplicated instrumentation; high cost	[69] [70]

* Signal to noise: S/N; Region of Interest: ROI. ^# Fluorescence Cross-Correlation Spectroscopy: FCCS.