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. 2022 Apr 29;23(9):4974. doi: 10.3390/ijms23094974

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(a) shows insulin-(green) and nNOS-(red)-immuno-positive cells in the islet of Langerhans of rats 1 and 2 weeks after induction of diabetes. No significant co-localization between nNOS and insulin was observed after the induction of diabetes in week 2. nNOS-immunoreactive ganglion cell (arrow) and nerve fibres (arrow head) were, however, observed in the exocrine pancreas. Scale bar = 10 μm. (b) shows morphometric analysis of insulin- and nNOS positive cells in the islet cells of the endocrine pancreas. Note the significant reduction in the number of nNOS-positive cells (** p < 0.01) and insulin-immunopositive cells (# p < 0.01) in pancreatic islets in week 2 compared to week 1. (n = 6).