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Mn-SOD activity as a function of culture age in the presence (+) or in the absence (−) of Mn2+ in the medium. The numbers at the top indicate the number of days of incubation. The Mn2+ concentration was 300 mg/liter. Samples of cell extracts from N-excess cultures were loaded (10 μg of protein per lane) onto a native PAGE gel and stained for Mn-SOD activity.
