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. 2022 May 4;23(9):5119. doi: 10.3390/ijms23095119

Figure 3.

Figure 3

Impact-induced chondrocyte cell death is Ucn receptor subtype-specific. (a) Representative confocal microscopy images of explants 72 h post-impact, pre-treated with agonists of CRF-R1 (Ucn-1 and CRF) or CRF-R2 (Ucn-1 and Ucn-2). Live cells were stained with CMFDA (green) and dead cells with propidium iodide (red). (b) Quantification of percentage cell death from impact experiments shown in (a) (n = 6 explants, 5 fields of view/explant; # = p < 0.01 vs. non-treated, * = p < 0.01 vs. impact). (c) Representative confocal microscopy images of explants 72 h post-impact, pre-treated with antagonists of CRF-R1 (CP) or CRF-R2 (Ast2B). Live cells were stained with CMFDA (green) and dead cells with PI (red). (d) Quantification of percentage cell death from impact experiments shown in (c) (n = 6 explants, 5 fields of view/explant; # = p < 0.05 vs. non-treated, * = p < 0.01 vs. impact, + = p < 0.05 vs. pre-impact CP).