Table 1.
Identification of phenolic compounds in Alder acorns a.
| Peak | Rt | λ (nm) | [M − H]−
(m/z) b |
MS/MS (m/z) b> |
Tentative Identification | Water Extract |
Ethanol Extract |
|---|---|---|---|---|---|---|---|
| 1. | 0.82 | 220 | 481.0628 | 300.9964/275.0187/257.0077/229.0715 | HHDP-hexoside(1-galloyl-2,3-HHDPl-α-glucose) | √ | |
| 2. | 0.92 | 324 | 355.0305 | 193.0130 | Ferulic acid hexoside | √ | |
| 3. | 1.07 | 222 | 481.0611 | 300.9986 | HHDP-hexoside | √ | |
| 4. | 1.16 | 273 | 649.0592 | 605.0702/479.0501/300.9987 | Trisgalloyl hexoside | √ | |
| 5. | 1.29 | 230 | 963.1341 | 933.0640/300.9985 | Methoxylated castalagin/vescalagin | √ | |
| 6. | 1.64 | 235/320 | 785.0615 | 633.0641/300.9984 | HHDP-digalloyl-hexoside | √ | |
| 7. | 1.71 | 274 | 783.0668 | 481.0624/300.9978 | Bis-HHDP-hexoside (pedunculagin isomer) | √ | |
| 8. | 1.95 | 280 | 483.0761 | 271.0187/193.0340/169.0134/125.0235 | Digalloyl-glucose | √ | |
| 9. | 2.34 | 274 | 783.0645 | 633.0770/481.0583/300.9964 | Bis-HHDP-hexoside (pedunculagin isomer) | √ | |
| 10. | 2.75 | 279 | 783.0702 | 481.0604; 300.9982 | Bis-HHDP-hexoside (pedunculagin isomer) | √ | √ |
| 11. | 3.30 | 235 | 633.0581 | 481.9926/300.9982 | HHDP-galloyl-hexoside | √ | |
| 12. | 3.65 | 326 | 367.0090 | 191.0128 | Methyl-caffeoyl-quinate | √ | |
| 13. | 3.66 | 274 | 783.0645 | 481.0613; 300.9984 | Bis-HHDP-hexoside (pedunculagin isomer) | √ | |
| 14. | 4.39 | 280 | 935.0596 | 783.0650; 633.0740 | Galloyl-bis-HHDP-hexoside | √ | √ |
| 15. | 4.5 | 235/320 | 785.0615 | 633.0641; 300.9984 | HHDP-digalloyl-hexoside | √ | √ |
| 16. | 4.82 | 325 | 633.0550 | 481.0470; 300.9982 | HHDP-galloyl-hexoside | √ | √ |
| 17. | 4.93 | 230 | 933.0406 | 915.0529; 633.0581; 450.9908; 301.0070 | Castalagin/vescalagin | √ | |
| 18. | 5.07 | 224 | 936.0582 | 300.9964 | Galloyl-bis-HHDP-hexoside | √ | |
| 19. | 5.32 | 279 | 783.0427 | 481.0117; 300.9964 | Bis-HHDP-hexoside (pedunculagin isomer) | √ | √ |
| 20. | 5.51 | 235 | 933.0645 | 450.9958; 301.0000 | Castalagin/vescalagin | √ | |
| 21. | 5.68 | 246 | 1869.1495 | 934.0719; 633.0584; 468.9935; 300.9985 | Dimer of galloyl-bis-HHDP-glucose (sanguiin H-6) | √ | √ |
| 22. | 5.85 | 230 | 933.0707 | 633.0404; 300.9991 | Castalagin/vescalagin | √ | |
| 23. | 6.17 | 252/364 | 433.0420 | 301.0354 | Ellagic acid pentoside | √ | √ |
| 24. | 6.5 | 255/365 | 300.9999 | 285.0425; 257.0208; 229.0137 | Ellagic acid | √ | √ |
| 25. | 6.87 | 225 | 935.0639 | 783.0604; 300.9987 | Galloyl-bis-HHDP-hexoside | √ | |
| 26. | 7.12 | 224 | 935.0799 | 783.0650; 300.9981 | Galloyl-bis-HHDP-hexoside | √ | |
| 27. | 7.56 | 350 | 447.0560 | 315.0143 | Isorhamnetin pentoside | √ | √ |
| 28. | 7.75 | 254/362 | 461.0121 | 300.9984 | Ellagic acid hexoside | √ | |
| 29. | 8.31 | 340 | 315.0133 | Isorhamnetin | √ | √ |
a Abbreviations: Rt, retention time; HHDP, hexahydroxydiphenyl; b Experimental data.