Table 3.
Dilution of Extract Stock Solution | The Percentage of Viability | |||
---|---|---|---|---|
A431 Cells | A549 Cells | |||
Ethanol Extract | Water Extract | Ethanol Extract | Water Extract | |
1:400 | 51 ± 2 f | 37 ± 2 f | 77 ± 5 f | 58 ± 1 f |
1:800 | 61 ± 2 f | 40 ± 1 f | 77 ± 3 f | 62 ± 1 f |
1:1600 | 68 ± 6 f | 38 ± 1 f | 80 ± 1 f | 67 ± 1 f |
1:3200 | 77 ± 2 f | 55 ± 3 f | 95 ± 1 | 71 ± 2 f |
Cells were incubated with indicated extract dilutions for 72 h, followed by determination of cells viability with MTT colorimetric method. The results are shown as percentage of cells’ viability versus control (cultures containing appropriate dilutions of the solvent; H2O or Ethanol) (mean values from three independent experiments). The percentage of viability was calculated in accordance with equation: % cell viability = [A extract/A control] × 100; where A extract—is the absorbance of the sample exposed to the extract of plants; A control—is the absorbance of the control sample—cell without extract exposition—100% cell viability. f for p < 0.05, versus Control probes.