Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 6;11(9):1572. doi: 10.3390/cells11091572

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

BNIP3 enhances mt-reactive oxygen species production, and attenuates mt-membrane potential and respiration, in cultured adult cardiomyocytes in vitro. (A,B). Representative fluorescent photomicrographs in cultured adult cardiac myocytes (ACM) transfected with adenovirus encoding for Null, BNIP3, or BNIP3 ShRNA (ShBNIP3), and loaded with the fluorophores TMRM and MitoSOX to assess mt-membrane potential and mt-reactive oxygen species, respectively. Live imaging was acquired at baseline and after 2 and 12 h of phenylephrine (PE, 10 μM) stress. Images are 20× magnified, scale bar 50 μm. Data were obtained from at least 3 independent experiments, and are presented as mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (C,D). Western blots show BNIP3 expression in normal (C) and in PE-stressed (D) ACM transfected with adenovirus Null (N), BNIP3 (B) or ShBNIP3 (ShB), * p < 0.05, ** p < 0.01, *** p < 0.001. (E). Representative tracings of mt-respiration by Oroboros oxygraph-2k system in permeabilized ACM transfected with adenovirus Null, BNIP3, or ShBNIP3. Data were obtained from 7 independent experiments and are presented as mean ± SD, * p < 0.05, ** p < 0.01, **** p < 0.0001. Abbreviations: F.I., fluorescence intensity; ns, not significant; Glu/Mal, glutamate/malate; ADP, adenosine diphosphate; Succ, succinate; Rot, rotenone; Oligo, oligomycin; FCCP, carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone; AA, Antimycin A; TMPD, N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride.

BNIP3 enhances mt-reactive oxygen species production, and attenuates mt-membrane potential and respiration, in cultured adult cardiomyocytes in vitro. (A,B). Representative fluorescent photomicrographs in cultured adult cardiac myocytes (ACM) transfected with adenovirus encoding for Null, BNIP3, or BNIP3 ShRNA (ShBNIP3), and loaded with the fluorophores TMRM and MitoSOX to assess mt-membrane potential and mt-reactive oxygen species, respectively. Live imaging was acquired at baseline and after 2 and 12 h of phenylephrine (PE, 10 μM) stress. Images are 20× magnified, scale bar 50 μm. Data were obtained from at least 3 independent experiments, and are presented as mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (C,D). Western blots show BNIP3 expression in normal (C) and in PE-stressed (D) ACM transfected with adenovirus Null (N), BNIP3 (B) or ShBNIP3 (ShB), * p < 0.05, ** p < 0.01, *** p < 0.001. (E). Representative tracings of mt-respiration by Oroboros oxygraph-2k system in permeabilized ACM transfected with adenovirus Null, BNIP3, or ShBNIP3. Data were obtained from 7 independent experiments and are presented as mean ± SD, * p < 0.05, ** p < 0.01, **** p < 0.0001. Abbreviations: F.I., fluorescence intensity; ns, not significant; Glu/Mal, glutamate/malate; ADP, adenosine diphosphate; Succ, succinate; Rot, rotenone; Oligo, oligomycin; FCCP, carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone; AA, Antimycin A; TMPD, N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride.