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Western immunoblots of LPS antigens reacted with anti-A-band LPS monoclonal antibody (A) and anti-B-band LPS monoclonal antibody (B). Lane 1, strain PAO1 (A+ B+); lane 2, strain AK1401 (A+ B−); lane 3, strain dps89 (A− B+); lane 4, strain rd7513 (A− B−). The blots confirmed that each strain produced the correct LPS chemotype.
