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Southern hybridization of chromosomal DNA from C. beijerinckii NCIMB 8052 and SA2. Chromosomal DNA was digested with HindIII and probed with ctfAB probe and ptb probe. Two X-ray films were overlapped when taking the picture. Lane 1, solvent-producing C. beijerinckii NCIMB 8052 culture; lane 2, degenerated C. beijerinckii NCIMB 8052 culture; lane 3, C. beijerinckii SA2 culture. SA2 is a butanol-tolerant degenerated isolate derived from C. beijerinckii NCIMB 8052 (3).
