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. 2022 Apr 26;14(9):2148. doi: 10.3390/cancers14092148

Figure 2.

Figure 2

Scoring interface to characterize spatial features of TIL infiltrates in breast cancer. Composite Tumor-TIL maps of H&E WSIs of breast cancer provide the ability to estimate the abundance and spatial distribution of TILs in a straightforward manner. Tumor-TIL maps are depicted as 4-panel composites containing low-resolution H&E WSI (upper left), tumor probability heatmap (upper right), lymphocyte probability heatmap (lower left), and the Tumor-TIL map (bottom right). Tumor and lymphocyte probability maps use a color scale to indicate probability from 0 (blue) to 1 (red). In the Tumor-TIL map, yellow represents tumor, red depicts lymphocytes, and non-tumor/non-lymphocyte patches are represented as gray background tissue. Observers use this interface to characterize the magnitude of intratumoral and peritumoral TIL infiltrates on a scale of 0 (none/absent) to 3 (marked). The presence of large intratumoral aggregates (forests), immune cold areas devoid of TILs (deserts), and tertiary lymphoid aggregates are indicated on the left panel. In this example, case 131, intratumoral strength was graded as 3 with weak/absent deserts and strong forests, peritumoral strength was graded as 3, and tertiary peritumoral aggregates as absent. Poor quality images and cases where the algorithms did not properly generate a Tumor-TIL map were flagged and excluded.