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Effect of α-linolenic acid on the viability of osteosarcoma MG63, 143B, and U2OS cells. Cell viability was then determined using an MTT assay. (A) MG63, (B) 143B, and (C) U2OS cells were treated with 0, 10, 20, 30, 40, 60, 80, 100, 150, and 200 μM α-linolenic acid for 24 h. The ratio of α-linolenic acid-treated cells to control cells was taken as the percentage of cell viability. The data are presented as the mean ± SD of three independent experiments. FASN, fatty acid synthase.
