Table 2.
Classical central nervous system (CNS) lesions associated with the presence of α-HVs consisted of meningeal mononuclear cell infiltrates (Me), lymphoplasmacytic perivascular cuffs (PC), diffuse microgliosis (M), intranuclear inclusion bodies (INIB), Malacia (Ma), neuronal necrosis and associated focal neurophagic nodules (NNs), and haemorrhages (H). OL: other lesions; C-I: co-infections; N.I.: nucleotide identity (with highest query cover); HSV-1: herpes simplex human type 1; α: alphaherpesvirus; γ: gammaherpesvirus; qPCR: quantitative polymerase chain reaction; Ct: threshold cycle; mTª: melting temperature; IHQ: immunohistochemistry; NA: not applicable. Highest nucleotide identities between sequences from our study are indicated in boldface.
| C. N° | CNS Lesions | GenBank Acc. No. | N.I. | qPCR | qPCR | IHQ | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Me | PC | M | INIB | Ma | NNs | H | OL | C-I | Ct | mTª | ||||
| 1 | - | X | X | - | - | - | - | - | - | EU003440 | 95.79% MN401208 (HSV-1) | NA | NA | - |
| 2 | X | X | X | - | - | X | - | - | DMV | KJ156330 | 99.45% GQ888669 (α) | NA | NA | - |
| 3 | X | X | X | X | X | X | X | - | - | KJ156331 | 99.49% KY680657(α) | 17.74 | 91 | + |
| 4 | X | X | X | X | X | X | X | - | - | KY680657 | 100% KY680656(α) | 7.32 | 90.50 | + |
| 5 | X | X | X | X | X | X | X | Choroiditis | - | KY680656 | 99.06% MN179658(α) | 23.31 | 90.00 | + |
| 6 | X | X | - | - | - | - | - | Choroiditis | - |
OM030214 OM030215 |
93.46% KP995683 (α)99.38% KM248274 (γ) |
32.15 | 88.00 | - |
| 7 | - | - | - | - | - | - | - | - | - | KY680659 | 90.37% JN863234 (α) | 12.91 | 86.50 | - |
| 8 | - | X | X | - | - | - | X | Choroiditis | S. aureus | KY680658 | 91.79% MG437204 (α) | - | - | - |
| 9 | X | X | X | - | - | X | - | - | DMV | MN179655 | 99.02% MG437212 (α) | - | - | + |
| 10 | X | X | X | X | - | X | - | - | - | MN179656 | 91.4% MG4337212 (α) | 22.20 | 91.50 | + |
| 11 | X | X | X | X | X | X | - | - | Brucella spp. | MN179657 | 92.56% MG437213 (α) | 22.81 | 90.50 | + |
| 12 | X | X | X | X | X | X | X | Choroiditis, syncytia | DMV | MN179658 | 99.06% KY680656(α) | 12.66 | 90.50 | + |