Figure 4.

ALDH1L2 knockout alters mitochondrial morphology. ALDH1L2 knockout led to a visible change to the morphology of the mitochoindria of the KO TS cells. Relative to control wt TS cells, the mitochondria of the KO TS cells have a larger network-like structure, where, in wide type TS cells, the mitochochondria are much more fragmented with an oval-like shape. Mitochondria in U251 tumor spheres and in adherent U251 cells were labeled with green fluroescent protein (GFP) fused to the leader sequence of E1 alpha pyruvate dehydrogenase and observed under a confocal microscope (see Supplemental Methods).