FIG. 3.

Western blot analysis of cyanobacterial proteins. Lanes 2 through 5 contained 8-μg portions of proteins extracted from the cyanobacterial cells harboring the shuttle vector pCB4 (lane 2) and the following vectors containing the pdc and adh genes: pCB4-Rpa (lane 3), pCB4-LRpa (lane 4), and pCB4-LR(TF)pa (lane 5). Proteins extracted from E. coli harboring pLOI295 containing the pdc and adh genes were used as a positive control (lane 1). The blots were probed with antiserum directed against Z. mobilis PDC protein and antiserum raised against Z. mobilis ADH II protein.